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. 2014 Dec;88(24):14268–14277. doi: 10.1128/JVI.02324-14

FIG 7.

FIG 7

Ad36 E4-ORF1 is closely related to Ad9 E4-ORF1, activates PI3K in a Dlg1-dependent manner, and elevates PI3K protein levels in MCF10A cells. (A) Ad36 E4-ORF1 elevates P-Akt and PI3K protein levels. Cell extracts from vector and 36ORF1 cells were prepared and analyzed in immunoblot assays. (B) HA-tagged Ad36 E4-ORF1 activates Akt in a PI3K-dependent fashion but not Erk1/2 and elevates PI3K proteins levels. Vector, HA-36ORF1, and rasV12 cells were treated with either a DMSO vehicle (−) or 100 μM LY294002 (LY) (+) for 30 min. Cell extracts were prepared and analyzed in immunoblot assays. (C) HA-tagged Ad36 E4-ORF1 activates PI3K by a Dlg1-dependent mechanism. Extracts of vector and HA-36ORF1 cells stably expressing either the Dlg1 shRNA (+) or the control scrambled shRNA (−) were prepared and analyzed in immunoblot assays.