FIG 2.

Determination of the linear enzymatic rate and sensitivity toward dsRNA for pOAS1 and OAS3. (A) Enzymatic activities were investigated for pOAS1 and OAS3 and plotted against the protein concentration. The enzymatic rate is displayed as the synthesis of 2-5A in nmol per min. The cutouts show an enlarged section of the linear interval of enzymatic rates with the best linear regression. (B) Sensitivity toward dsRNA was investigated using fixed concentrations of 2 nM OAS3 (circles) and 6 nM pOAS1 (squares) plotted against increasing amounts of poly(I·C). The experimental data were analyzed by nonlinear regression using the model indicated in Materials and Methods.