FIG 5.

The MVA-dsneo-ΔB15 and MVA-dsEGFP-720(o) mutants induce PKR activation. Murine A31 cells were infected at day 1 after seeding with crude stocks of the indicated viruses at an MOI of 10. Phosphorylation of eIF2α (p-eIF2α) in cell lysates prepared after the indicated times of infection was analyzed by immunoblotting using an antibody against the phospho-epitope Ser-51 of eIF2α (“p-eIF2α” panels). Loading was monitored by stripping the blots and reprobing with an antibody against murine β-tubulin.