FIG 6.

Midpoint IgM and IgG titers elicited in sera by immunization with the hyperglycosylated cores. (A) Midpoint IgM titers of groups A to D. ELISA plates were coated with each respective “self” immunogen as the target protein. The antisera from weeks 0, 2, 6, 10, 14, and 18 were then added to the plates at an initial dilution of 1:10, followed by 5-fold serial dilutions. (B) Midpoint IgG titers of groups A to D (left), as well as groups F to I, which were boosted with YU2 gp140-F trimers (right). ELISA plates coated with the respective immunogens were incubated with 5-fold serial dilutions of the sera, starting with a 1:200 dilution. (C) Comparative IgG and IgM immune responses of the hyperglycan antisera against 0G. Plates coated with 0G were incubated with 1,000-fold-diluted sera from animal groups inoculated with either 0G, 3G, 6G, or 7G, followed by HRP-conjugated anti-rabbit IgG or IgM for detection. The bleed time points, in weeks, are denoted on the x axis, with the arrowheads marking the inoculation time points. O.D., optical density.