FIG 7.
B cell response to URT HSV-1 infection. (a) BALB/c mice were infected i.n. (5 μl; no anesthesia) with influenza virus A/X31 (3 × 106 PFU), HSV-1 (3 × 106 PFU), or MuHV-4 (105 PFU). Ten days later, virus-specific antibodies were quantitated by ELISA in sera and nasal washes of 3 mice per virus. Each line shows the absorbance curve for 1 mouse. Dilutions were from 1/100 for sera and from 1/2 for nasal washes. These maximal concentrations are plotted as 100%. (b) The same mice, infected with MuHV-4 (M), influenza virus (F), or HSV-1 (H), were analyzed for virus-specific AFCs by ELIspot. Each bar shows the mean ± SEM for 6 mice. Influenza virus elicited significantly more NALT AFCs than did MuHV-4 or HSV-1 for all isotypes (P < 0.05) and significantly more IgA+ AFCs at all sites (P < 0.001). Other differences were insignificant or inconsistent across sites.