FIG 7.

Representative field of dually labeled PRV particles from the cytoplasm of Vps4A-EQ-expressing cells. PNS samples prepared from PRV-infected Vps4A-EQ-expressing cells (Pre Tet- conditions) were flowed into a microchamber precoated with green fluorescent microtubules. (A to D) Representative field of PRV particles viewed in the blue (A), green (B), or red (C) channel or merged (D). (E) Magnification (×2.7) of the region boxed in panel D. Red arrowhead indicates PRV capsid fluorescing only in the red channel, purple arrowhead indicates gD-TagBFP-positive red-fluorescing capsid, and white arrowhead indicates red-fluorescing capsid colocalizing with gD-TagBFP and Vps4A-EQ-GFP. Scale bars in all panels represent 10 μm.