TABLE 1.
Quantitation of particle numbers per microscopic field for PNS samples prepared from HSV- and PRV-infected cells
| Virus/Vps4 allele | No. of particles (mean ± SD) of typea: |
||
|---|---|---|---|
| mRFP1+ | mRFP1+ GFP+ | Motile mRFP1+ | |
| Pre Tet- conditions | |||
| HSV/Vps4A | 365 ± 18 | 29 ± 4 | 20 ± 2 |
| HSV/Vps4A-EQ | 319 ± 25 | 72 ± 6 | 7 ± 1 |
| PRV/Vps4A | 204 ± 42 | 24 ± 5 | 13 ± 2 |
| PRV/Vps4A-EQ | 189 ± 21 | 67 ± 7 | 7 ± 1 |
| Pre Tet+ conditions | |||
| HSV/Vps4A | 291 ± 49 | 16 ± 4 | 17 ± 4 |
| HSV/Vps4A-EQ | 180 ± 39 | 42 ± 15 | 4 ± 1 |
a
Microscopic fields from 8 HSV (Pre Tet-), 8 HSV (Pre Tet+), and 12 PRV (Pre Tet-) chambers prepared independently were counted to determine mean particle number per field. mRFP1+, capsid-associated particles; mRFP1+ GFP+, capsid-associated particles that colocalized with Vps4A or Vps4A-EQ; motile mRFP1+, capsid-associated particles that became motile upon the addition of ATP.