FIG 5.

Recognition of known Mamu-B017-restricted CTL epitopes identified in rhesus macaques by Mane-B017⁺ pig-tailed macaques. (A and B) The SIV sequences of 2 CTL epitopes previously identified in Mamu-B017⁺ rhesus macaques (Nef IW9 and Env FW9) were studied in Mane-B017⁺ pig-tailed macaques and compared to common mutations observed in Mamu-B017⁺ rhesus macaques. The mutations identified are underlined and in bold. (C) Identification of an Env FW9/Mane-B017 tetramer-specific CD8 T cell population in PBMCs from a Mane-B017⁺ pig-tailed macaque. PBMCs were unstimulated or stimulated for 10 days with the Env epitope, and gated CD3⁺ CD8⁺ lymphocytes were stained with an Env FW9/Mane-B017 tetramer.