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Temperature-dependent expression of FdeC. (A) β-Galactosidase activities (in Miller units) of N39lacIZ (control) and N39fdeC::lacIZ grown at various temperatures. The fdeC promoter was active only at 39°C and 43°C. (B) Western blot analysis of STEC strains N39, N39fdeC, and EDL933 grown at 28°C, 37°C, 39°C, and 43°C. As in the reporter assay, FdeC expression was detected only at or above 39°C.
