TABLE 2.
Comparison of different enzymes for preparation of (S)- or (R)-DmCpCa
| Enzyme | Substrate load (mM) | Time (h) | Temp (°C) | ees (%)b | eep (%) (R or S preference) | Conversion rate (%) | E value | Reference |
|---|---|---|---|---|---|---|---|---|
| Novozyme 435 | 65 | 64 | 30 | 34.0 | 99.0 (S) | 25.6 | 278 | 34 |
| Lipozyme IM | 65 | 20 | 30 | 1.9 | 65.9 (S) | 2.8 | 5 | 34 |
| Lipase PS-D | 65 | 20 | 50 | 97.8 | 85.0 (R) | 53.5 | 55 | 34 |
| Lipase F-AP15 | 65 | 20 | 40 | 98.3 | 85.0 (R) | 53.6 | 58 | 34 |
| Lipase L-1754 | 65 | 20 | 40 | 95.0 | 79.0 (R) | 54.7 | 31 | 34 |
| RhEst1 | 500 | 21 | 30 | 97.7 | 97.5 (S) | 47.8a | 240 | This work |
a
Molar isolation yield.
b
ees, enantiomeric excess of substrate.