FIG 3.

Rates of AQ conversion by cell extracts of Rhodococcus sp. BG43 (μM AQ converted per hour and mg total protein). Desalted crude cell extracts (2 mg protein/ml) were incubated with 20 μM HHQ and 500 μM NADH or with 20 μM PQS. White bars represent extracts from cells grown in LB medium, and gray bars represent extracts from LB cultures supplemented with PQS 2 h prior to harvesting. Data represent mean values from two independent biological replicates ± standard errors. Cell extracts treated for 10 min at 99°C did not support AQ conversion.