TABLE 2.
Plasmids used in this study
| Plasmid | Relevant features | Source or reference |
|---|---|---|
| pUC18 | Cloning vector, Apr, 2.7 kb | Invitrogen |
| pUC18-GlpKF | pUC18 derivative carrying the GlpF and GlpK genes from E. coli JM109 | This study |
| pUC18-GlpKFD | pUC18 derivative carrying the GlpF, GlpK, and GlpD genes from E. coli JM109 | This study |
| pUC18-GlpKFD-TPI | pUC18 derivative carrying the GlpF, GlpK, GlpD, and TPI genes from E. coli JM109 | This study |
| pUC18-GlpKFD-TPI-YqhD | pUC18 derivative carrying the GlpF, GlpK, GlpD, TPI, and YqhD genes from E. coli JM109 | This study |
| pCER-EGFP | Lactobacillus panis PM1 expression vector, in which the EGFPa gene was expressed under the control of PorfX promoter, Emr Cmr, 6.0 kb | 14 |
| pCER-GlpKFD-TPI | pCER-EGFP derivative, in which the EGFP gene was replaced with the GlpKFD-TPI fragment, Emr Cmr, 10.7 kb | This study |
| pCER-GlpKFD-TPI-YqhD | pCER-EGFP derivative, in which the EGFP gene was replaced with the GlpKFD-TPI-YqhD fragment, Emr Cmr, 11.9 kb | This study |
a
EGFP, enhanced green fluorescent protein.