TABLE 3.
Effects of acetic acid on the NADH recycling systema
| Acetic acid content in mediumb | OD600 | Glycerol consumed (mM) | End product concn (mM) |
||
|---|---|---|---|---|---|
| Lactic acid | Acetic acid | Ethanol | |||
| 50 mM | 1.53 ± 0.07 | 57.24 ± 0.77 | 46.15 ± 0.38 | 29.80 ± 0.30c | 29.02 ± 0.28 |
| None | 0.59 ± 0.01 | 32.79 ± 0.53 | 26.05 ± 0.89 | 6.07 ± 0.08 | 11.70 ± 0.08 |
a
Values are means ± standard errors.
b
Strain PM1-pCER-glpFKD-TPI-YqhD was cultured in mMRS medium containing 160 mM glycerol with or without acetic acid for 24 h under pH-uncontrolled conditions.
c
The initially available acetic acid amount (50 mM) was reduced to 29.80 mM.