TABLE 4.
Effects of pH on the NADH recycling systema
| Initial medium pHb | Glycerol consumed (mM) | End product concn (mM) |
||
|---|---|---|---|---|
| Lactic acid | Acetic acidc | Ethanol | ||
| 6.5 | 59.47 ± 0.23 | 56.19 ± 0.31 | 22.62 ± 0.46 | 37.77 ± 1.65 |
| 4.5 | 20.59 ± 0.46 | 14.98 ± 0.08 | 46.04 ± 0.56 | 5.14 ± 0.08 |
Values are means ± standard errors.
Strain PM1-pCER-glpFKD-TPI-YqhD was cultured in mMRS medium containing 160 mM glycerol for 24 h under pH-uncontrolled conditions.
The initially available acetic acid amount under each pH condition was 50 mM.