TABLE 2.
Oligonucleotide primers used for the M. californicum MLVA
| Primera | Sequence (5′→3′) | Locationb | PCR product size (bp) |
|---|---|---|---|
| MCTR569_F | CACTAAACTCAGTAACAG | 568810–568827 | 107 + total size of repeat units |
| MCTR569_R | TGAAAAAGAGAAGACCG | 570262–570278 | |
| MCTR695_F | GATGATAAGTCTGCTTGTC | 695021–695039 | 346 + total size of repeat units |
| MCTR695_R | TTTTCATAGTCCGAACTGG | 696266–696284 | |
| MCTR698_F | TTTATCTCCGATTGCGTC | 697457–697474 | 256 + total size of repeat units |
| MCTR698_R | TGAAGCACTTTGTTGTCC | 698205–698222 | |
| MCTR701_F | CAACATTGCCGTTACTTGC | 701113–701131 | 347 + total size of repeat units |
| MCTR701_R | CTTAGGACCTTCCAATTCG | 702359–702377 |
a
F, forward primer; R, reverse primer.
b
Position in the M. californicum HAZ160_1 genome sequence (accession no. AP013353).