FIG 1.

Insertional inactivation of codY in the Clostridium botulinum ATCC 3502 strain. (A) PCR analysis of codY mutation. Ll.LtrB-derived intron insertion was detected with primers flanking the insertional site yielding a 2.2-kb product in the codY mutant. M, Roche DNA molecular weight marker VII. (B) Southern blot analysis of HindIII-digested DNA from the WT, the pMTL007-C-E2 plasmid, and the codY mutant with intron-specific probe. (C) Western blot analysis of CodY expression in WT and codY mutant cultures.