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Expression of botA in the Clostridium botulinum ATCC 3502 wild-type strain and the codY mutant. RNA was isolated after 5, 6 (mid-exponential phase), 9 (transition phase), and 13 (early stationary phase) h of growth and analyzed for relative botA expression using RT-qPCR. Target gene expression was normalized to 16S rrn and calibrated to the WT at 5 h. Error bars represent standard deviations from three biological replicates. *, P < 0.05 (Student's t test).
