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Neurotoxin production by Clostridium botulinum ATCC 3502 wild-type strain and codY mutant. ELISA analysis of botulinum neurotoxin in WT and codY mutant culture supernatants after 5, 6, and 9 h (left) and 13, 24, 48, and 96 h (right) of growth. Error bars indicate standard deviations from three biological replicates. *, P < 0.05; **, P < 0.01 (Student's t test).
