TABLE 2.

Primer pairs used to screen for the presence of the arg, nos, and otc genes in Staphylococcus strains

PCR target	Primer pair	Nucleotide sequences (forward and reverse, respectively) (5′ to 3′)	Amplicon size (bp)	Species	ENA accession no. of the entries used for primer design
arg	ARG-F and ARG-R	THGGDATGAGRGATTTRGA and GCAAARTGRCTTTCHCKATA	247	S. caprae, S. capitis, S. epidermidis, S. saprophyticus, S. simiae, S. warneri, S. xylosus	EFS16894, BAE17866, ABF13437, EEE48798, EES42258, EEQ79567, EHJ09071, EGG96003, EGS40237
nos	NOS-1-F and NOS-1-R	CAAAAATGGCATGGAGAAAC and CCATCCATTAAAAGGTGCTG	523	S. capitis, S. epidermidis, S. equorum, S. warneri	EEE48709, EEQ78851, EFA89185, EFV88123, EGG97234, EGG63344, EGG71090 EGG71063, EGS40499, EGS80208, EGS78236, EGS80733, EHM67228, EHQ72179, EHQ76423, EHQ75636, EHR79954, EHR81536, EHR82566, EHR90879, EHR89224, EHR94024, EHR95252, EHR98881, EHS00755, EHQ81520, EEE48709, EEQ78851, EFA89185, EFV88123, EGG97234, EGG63344, EGG71090, EGG71063, EGS40499, EGS80208, EGS78236, EGS80733, EHM67228, EHQ72179, EHQ76423, EHQ75636, EHR79954, EHR81536, EHR82566, EHR90879, EHR89224, EHR94024, EHR95252, EHR98881, EHS00755, EHQ81520, EJE31238, EJE36529, EJE38416, EJE39628, EJE43873, EJE45333, CCI58543
	NOS-2-F and NOS-2-R	GGNGATCCTGCTGAAAAAGA and GCAGCGGTNAAATGATCNAC	490	S. aureus, S. carnosus	CAL28392, AAW36946
	NOS-3-F and NOS-3-R	TGGACGCTTATTTTGGGATT and GGGGCTAACCAAGACCATTT	768	S. haemolyticus	BAE04347
	NOS-4-F and NOS-4-R	TTGGATGGCAAGGTCAGTAT and ACCAATGTGGGAGACAAAGA	541	S. saprophyticus subsp. saprophyticus	BAE18022, EHY92989
otc	OTC-1-F and OTC-1-R	AAACATGTTATTGTAAACATTTTGTC and CACACAGGCACACCTGAATA	594	S. haemolyticus	AP006716
	OTC-2-F and OTC-2-R	ACGCCGGTATAGAACAGCAAAAA and CGTCAGGTTCACCCATTGATACC	618	S. haemolyticus	AP006716
	OTC-3-F and OTC-3-R	CCCTTAAAGAATTAAATCCAACAGAT and GATTACAACTTCATAACCTTTTTGG	586	S. haemolyticus	AP006716