FIG 2.

Time-dependent effect of C. difficile RT017 CF5 and M68 on BMDC cytokine mRNA expression. BMDCs were stimulated with CF5 and M68 at an MOI of 10, and the mRNA expression of IL-12 family members—p35 (A), p40 (B), p19 (C), p28 (D), EBI3 (E) IL-10 (F), and IL-1β (G)—was quantified by real-time PCR. The data are presented as the fold increase compared to expression in uninfected control cells. The data represent the means ± the SEM from three independent experiments. **, P < 0.01; ***, P < 0.001 (significant interstrain differences). P values were obtained by using ANOVA with a Bonferroni posttest analysis.