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Cultivation and PCR evaluation of B. burgdorferi-infected Ixodes ticks
| Tick stage | B. burgdorferi strain | No. of ticks positive/total no. (%) |
||
|---|---|---|---|---|
| Culture | qPCR | pncA complementation | ||
| Larva | MSK5 | 9/9 (100) | 9/9 (100) | 8/9 (89) |
| ML23/pBBE22 | 2/20a (10) | 8/20a (40) | 6/20a (30) | |
| ML23/pJH420 | 9/20a (45) | 8/20a (40) | 6/20a (30) | |
| Nymph | MSK5 | 10/10a (100) | 10/10 (100) | 9/10 (90) |
| ML23/pBBE22 | 0/20a (0) | 4/20a (20) | 1/20a (5) | |
| ML23/pJH420 | 0/20a (0) | 5/20a (25) | 0/20a (0) | |
Total for ticks from mouse 1 and mouse 2.
