FIG 2.

FTS_1680 contributes to the intracellular survival in BMMs and J774.2 cells. The growth kinetics of the wild-type (filled circles), inFTS_1680 (filled squares), inFTS_1680 complemented (filled triangles), inFTS_0201 (empty squares), and inFTS_0778 (empty circles) strains inside murine bone marrow-derived macrophages (BMMs) (A) or J774.2 cells (B) are shown. The number of intracellular bacteria was determined at 1, 6, 12, and 24 h postinfection. Results are shown as the average log₁₀ CFU per well ± SD for three independent experiments performed in triplicate (n = 9). Statistical significance was analyzed using an unpaired t test; *, P < 0.05; **, P < 0.01; ***, P < 0.001 (comparing inFTS_1680 with the wild-type FSC200 strain). #, P < 0.05 (comparing the complemented strain with inFTS_1680).