TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source and/or reference |
|---|---|---|
| E. coli strains | ||
| α-Select Gold Efficiency | F− deoR endA1 recA1 relA1 gyrA96 hsdR17 (rK− mK+) supE44 thi-1 phoA Δ(lacZYA argF)U169 ϕ80lacZΔM15 λ− | Bioline |
| DHM1 | F′ cya-854 recA1 endA1 gyrA96 (Nalr) thi-1 hsdR17 spoT1 rfbD1 glnV44(AS) | BACTH system kit; Euromedex (46) |
| Francisella strains | ||
| FSC200 | F. tularensis subsp. holarctica, clinical isolate | Francisella strain collection (18) |
| SchuS4 | F. tularensis subsp. tularensis | USAMRIID strain collection |
| inFTS_0201 | FSC200 inFTS_0201 | This study |
| inFTS_0778 | FSC200 inFTS_0778 | This study |
| inFTS_1680 | FSC200 inFTS_1680 | This study |
| inFTS_1680 complemented | inFTS_1680 with pKK289FTS_1680 | This study |
| inFTT_0166c | SchuS4 inFTT_0166c | This study |
| Plasmids | ||
| pCR4-TOPO | Cloning vector, Ampr Kmr | Invitrogen |
| pKEK1140 | F. tularensis-specific TargeTron plasmid | 20 |
| pKEK1140/in0201 | pKEK1140 with FTS_0201 inserted | This study |
| pKEK1140/in0778 | pKEK1140 with FTS_0778 inserted | This study |
| pKEK1140/in1680 | pKEK1140 with FTS_1680 inserted | This study |
| pKEK1140/in0166c | pKEK1140 with FTT_0166c inserted | This study |
| pKK289gfp | Ft ori, p15a ori, Kmr, groES promoter | 21 |
| pKK289FTS_1680 | pKK289 with intact FTS_1680 | This study |
| pKNT25 | BACTH system plasmid | 46 |
| pUT18 | BACTH system plasmid | 47 |
| pUT18/1680 | pUT18 with intact FTS_1680 | This study |
| pKNT25/1670 | pKNT25 with intact FTS_1670 | This study |
| pKNT25/1167 | pKNT25 with intact FTS_1167 | This study |
| pKNT25/1166 | pKNT25 with intact FTS_1166 | This study |
| pKNT25/0277 | pKNT25 with intact FTS_0277 | This study |
| pKNT25/0263 | pKNT25 with intact FTS_0263 | This study |