FIG 2.

Phylogenetic analysis of full-ORF tprK DNA sequences from secondary lesions. A representative example of phylogenetic relationships (a maximum-parsimony cladogram) among the inoculum and the individual secondary lesions (identified by rabbit number and lesion letter) that developed in rabbit 7901 is shown. Lesion 7901B did not contain amplifiable treponemes. The clusters enclosed by shaded ovals correspond to the sequences from 10 plasmid clones per lesion. Bootstrap values of the major branches are shown, demonstrating separation of the lesion sequences from the inoculum sequence. Note that the sequences for each lesion are in tight clusters, consistent with a single-cell origin for each lesion.