FIG 2.

Inflammatory responses in BMDCs stimulated with killed bacteria. TNF-α (A) and NO (C) production was measured in wild-type C57BL/6 BMDCs stimulated with 0.1, 1, 5, 10, or 50 μg/ml of WT-COH1 or ermCOH1 heat-killed bacteria or with LPS (100 ng/ml), used as a control. TNF-α or NO was measured in supernatants collected after 24 h. (B) Kinetics of TNF-α induction in BMDCs stimulated with 1 μg/ml of WT-COH1 or ermCOH1 heat-killed bacteria. TNF-α was measured in supernatants collected at the indicated times after stimulation. Statistical evaluation was performed by the Student t test. ***, P < 0.001; **, P < 0.01; *, P < 0.05. Data shown are means plus SD of triplicate observations determined in one experiment and are representative of three independent experiments.