FIG 5.

(A) Accumulation of autophagy markers (modulation of the autophagy) in the presence of Omp33-36. The accumulation of p62, LC3B, and caspase 8 was analyzed by Western blotting in wt MEFs. Cells incubated with 8 μg/ml of Omp33-36 were tested at 0, 8, 16, and 24 h. (B, D) Inhibition of autophagy before formation of autophagosomes. Immunoblotting was performed to determine the levels of p62 and LC3B in wt MEFs in the presence of 1 μM wortmannin (WM), which was added at 0, 8, 16, and 24 h (*), in the presence and absence of 8 μg/ml of Omp33-36. (C, E) Inhibition of autophagy and accumulation of autophagosomes. Western blotting was used to study the autophagy process by determining p62 and LC3B levels in the presence of 0.1 μM bafilomycin (BAF), which was added at 0, 8, 16, and 24 h (*), in the presence and absence of 8 μg/ml of Omp33-36.