FIG 2.

MHC tetramer and corresponding LacZ-inducible reporter cell line for the F4 epitope. (A) Naive splenocytes were pulsed with five previously reported blood stage CD8⁺ T cell epitopes, Pb1, or Pb2 and incubated with CD8⁺ T cells from PbA-infected mice to perform an IFN-γ ELISpot assay. *, P < 0.05; ***, P < 0.001; ****, P < 0.0001 (one-way ANOVA with Dunnett's posttest). (B) Splenocytes from PbA-infected mice were labeled with pMHC tetramers generated from each of the 5 peptides. Only F4-specific CD8⁺ T cells were stained (lower panel); the upper panel shows D5 tetramer staining, and the results for A6, F6, and G4 are similar. (C) LR-WH3.4 cells transduced with the TCR pair boldfaced in Table 1 were incubated with unpulsed (higher panel) or F4-pulsed (lower panel) EL4 cells, and X-Gal staining was performed.