FIG 3.

Pb2- and F4-specific CD8 T cells are induced during Plasmodium infection. Leukocytes from spleens and brains of C57BL/6 mice infected with different parasite species at day 7 postinfection were isolated and stained with live/dead stain, antibodies, and Pb2 and F4 tetramers in PE and BV421 fluorophores. (A) Representative dot plots of CD8⁺ T cells (gated on live CD8a⁺ CD16/32⁻ cells) in the spleens and brains of mice. (B and C) The numbers of tetramer-positive CD8⁺ T cells from each organ for Pb2 (B) and F4 (C) are presented. **, P < 0.01, Mann-Whitney U test. Bars represent medians. The results of two independent but identical experiments are pooled.