FIG 5.
Only brain microvessels from mice infected with ECM-causing PbA parasites cross-present malaria epitopes. Brain microvessels from the brains of infected mice at day 7 were isolated and cultured with LR-BSL13.6b (A) or LR-WH3.4a (B) reporter cell lines recognizing H-2Kb MHC complexed with Pb2 and F4 peptides, respectively. X-Gal staining was performed on the cell culture after overnight incubation, and the numbers of blue cells were determined. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (one-way ANOVA with Bonferroni's multiple-comparison test on log-transformed data). Bars represent means.