TABLE 1.
Clone | TRAV | TRAJ | TCRα junction | TRBV | TRBD | TRBJ | TCRβ junction |
---|---|---|---|---|---|---|---|
A18 | 13-4/DV7 | 52 | CAMALGANTGKLTF | 12-1 | 1 | 2-1 | CASFTSNYAEQFS |
A19 | 13-4/DV7 | 52 | CAMALGANTGKLTF | 12-1 | 1 | 2-1 | CASFTSNYAEQFF |
A20 | 13-4/DV7 | 52 | CAMALGANTGKLTF | 12-1 | 1 | 2-1 | CASFTSNYAEQFF |
A21 | 1 | 1 | 1-1 | CTCSGTEVFF | |||
A23 | 16N | 6 | CAMRAPGGNYKPTF | 20 | 1 | 2-2 | CGASRDRNTGQLYF |
A24 | 20 | 1 | 2-2 | CGASRDRNTGQLYF |
F4 tetramer-specific CD8+ T cells were sorted from the spleens of PbA-infected mice and subjected to single-cell TCR sequencing. The TCRαβ pair in boldface was selected for transduction into LR-Ø cells to create the LR-WH3.4 reporter cell line.