TABLE 2.
TCR sequences of brain-sequestered CD8+ T cells isolated from mice suffering from ECMa
| Cell | TRAV | TRAJ | TCRα junction | TRBV | TRBJ | TRBD | TCRβ junction |
|---|---|---|---|---|---|---|---|
| 13.6 | 9N-2 | 44 | CVLSRVGSGGKLTL | 12-2 | 1-1 | 1 | CASSLRGRDTEVFF |
| 13.20 | 12-2 | 1-1 | 1 | CASSLRGRDTEVFF | |||
| 11.76 | 7-5 or 7D5 | 23 | CAVSGYNQGKLIF | 26 | 2-1 | 2 | CASSLGGRAEQFF |
| 13.10 | 7-5 or 7D5 | 23 | CAVSGYNQGKLIF | 26 | 2-1 | 2 | CASSLGGRAEQFF |
| 11.80 | 13D-1 | 18 | CAVPRDRGSALGRLHF | 26 | 2-1 | 2 | CASSLGNYAEQFF |
| 13.5 | 26 | 2-1 | 2 | CASSLGNYAEQFF | |||
| 11.84 | 7-3 | 40 | CAVIFTGNYKYVF | 12-2 | 2-7 | 1 | CASSLGDEQYF |
| 13.44 | 7-3 | 40 | CAVIFTGNYKYVF | 3 | 2-5 | 1 | CASSLGDTQYF |
| 13.33 | 16 | 1-1 | 1 | CASSSGTGNTEVFF | |||
| 10C11 | 6-7 | 50 | CALSDRSSSFSKLVF | 16 | 1-1 | 1 | CASSSGTGNTEVFF |
| 11.3 | 13-3 | 2-5 | 1 | CASSPGQGTDTQYF | |||
| 11.39 | 4-2 | 16 | CALSDRSSSFSKLVF | ||||
| 10C6 | 4-2 | 16 | CALSDRSSSFSKLVF | 13-3 | 2-5 | 1 | CASSPGQGTDTQYF |
a
TCRs from single CD8+ T cells isolated from the brains of PbA-infected mice were sequenced and analyzed with IMGT/V-QUEST; shown here are those that appear to be conserved. Paired TCRα and β sequences could not be obtained for some clones. Boldface, potentially conserved sequence pairs used for transduction into reporter cells.