FIG 2.
Stx2-mediated detection of Gb3Cer and Gb4Cer in neutral GSL preparations from Vero and T24 cells. (A to C) GSLs in amounts equivalent to those from 5 × 105 cells were applied (lanes b, Vero cells; lanes c, T24 cells). GSL reference mixture 1, composed of Gb4Cer as the predominant GSL and Gb3Cer in a lower abundance (lanes a), and reference mixture 2, containing equimolar amounts of Gb3Cer and Gb4Cer (lanes d), were present at 2 μg and 1 μg, respectively. Supernatants of EHEC isolates UR5703/202 (Stx2a) (A), UR5779/201 (Stx2a) (B), and UR131 (Stx2b) (C) were investigated.