FIG 3.

Positive-ion-mode ESI–Q-TOF mass spectra of antibody-detected Gb3Cer species from T24 cells. (A) Overview of the MS¹ spectrum obtained from the silica gel extract of the TLC overlay assay (shown in the inset) performed with an aliquot of GSL at an amount corresponding to the amount from 1 × 10⁶ cells. The area comprising the immunostained double band, from which the silica gel was scraped off, is indicated by a dotted rectangle. (B) Low-energy CID MS² spectrum of monosodiated Gb3Cer (d18:1, C_24:0) with m/z 1,158.80. (C) Molecular structure and explanatory fragmentation scheme of Gb3Cer (d18:1, C_24:0). arb., arbitrary.