TABLE 1.
Characteristics of EHEC isolates from UTI casesa
| Characteristic | Value or result for strain: |
||
|---|---|---|---|
| UR5703/202 | UR5779/201 | UR131 | |
| Age (yr) | 69 | 22 | 44 |
| Disease | Cystitis | Cystitis | Hemorrhagic cystitis |
| Serotype | O145:H− | Ont:H− | O76:H19 |
| ST, CC | ST32, CC32 | ST330, CC10 | ST675 |
| Phylogenetic group | A | E | B1 |
| Presence or type of toxin genes | |||
| cdt I, II, III, IV, V | − | − | − |
| astA | − | − | − |
| α-hlyA | − | − | − |
| cnf1, cnf2 | − | − | − |
| cif | + | − | − |
| vat | − | − | − |
| estIA | − | + | − |
| sat | − | − | − |
| EHEC hlyA | + | + | + |
| espP subtype | α-espP | γ-espP | − |
| stx genotype | stx2a | stx2a | stx1c + stx2b |
| Presence or type of adhesin genes | |||
| fimH | + | − | + |
| papA | − | − | − |
| sfa-foc | − | − | − |
| eae subtype | eae γ | eae ι-2 | − |
| bfpA | − | − | − |
| iha | + | − | − |
| saa | − | − | + |
| efa1 | + | − | − |
| sfpA | − | − | − |
| paa | + | − | − |
| aap | − | − | − |
| pet | − | − | − |
| aggA | − | − | − |
| aafA | − | − | − |
| agg3A | − | − | − |
| hdaA | − | − | − |
| aaf5A | − | − | − |
| Presence or type of other genes | |||
| irp2 | − | + | − |
| fyuA | − | + | − |
| chuA | + | + | − |
| tir subtype | EHEC tir | + (nt) | − |
| cdiAB | − | − | + |
| escV | + | + | − |
| espF | + | + | − |
| espG | + | − | − |
| map | + | + | − |
| terE | + | + | − |
| ureD | + | + | − |
| Presence of plasmid genes | |||
| etpD | − | − | − |
| katP | − | − | − |
| traT | − | + | + |
| Phenotypes | |||
| Fermentation of sorbitol | + | + | + |
| Growth on CT-SMAC | + | + | − |
| EHEC Hly expression | + | + | + |
| Stx Vero cell titer | 128 | 256 | 256 |
| Stx T24 cell titer | 8 | 8 | 32 |
| Production of colicin | − | − | + |
| Production of aerobactin | − | − | − |
| Motility | − | − | + |
| Adherence pattern | |||
| HEp-2 cells | Weak, undefined | Weak, undefined | LA |
| T24 cells | Weak, undefined | Weak, undefined | LA |
a
All cases occurred in females. ST, sequence type; CC, clonal complex; LA, localized adherence; nt, nontypeable.