FIG 6.

Identification of glycosylation sites. OmpA-like proteins isolated from the P. gingivalis wild-type strain by WGA column chromatography were subjected to LC-MS/MS analysis to identify the glycosylation sites. Tryptic peptides from corresponding SDS-PAGE gel bands were analyzed. Peptide sequences and posttranslational modifications were determined using the SEQUEST and DTASelect programs. (A) Amino acid sequences of Pgm6 and Pgm7. Boldface letters indicate peptides identified by MS, and the underlined peptide is glycosylated. Peptides not identified by MS are indicated in lightface. The sequence coverage of Pgm6 and Pgm7 was 58.3% and 55.5%, respectively. (B) Detection of glycosylation sites in Pgm6. Modified amino acids are underlined.