FIG 7.

Binding of OmpA-like glycoproteins from P. gingivalis to extracellular matrix (ECM) molecules. Purified P. gingivalis OmpA-like proteins were applied to a polystyrene plastic plate coated with ECM molecules and incubated at room temperature for 2 h and then at 4°C overnight. After being washed, the wells were treated with an anti-Pgm6/7 antibody (1:10,000), followed by peroxidase-conjugated goat anti-rabbit IgG (1:5,000). EIA peroxidase substrate, 3,3′,5,5′-tetramethylbenzidine, was then added to each well. The reactions were stopped by the addition of 100 μl of 0.5 M H₂SO₄. The binding activity was assessed by measuring the OD₄₅₀ values in a microplate reader. All assays were performed in triplicate, and the standard deviations were determined. *, significantly different from Pgm6/7 (P < 0.05).