FIG 9.

Effect of mutating glycosylation-related genes on the glycosylation of WGA-reactive OmpA-like proteins derived from P. gingivalis. Whole-cell lysates and the WGA lectin-bound fraction derived from P. gingivalis wild-type and mutant strains were subjected to SDS-PAGE under reducing conditions and stained with CBB or Pro-Q emerald. The following strains were used: ATCC 33277 (wild type), Prfa (rfa), PugdA (ugdA), RE1 (gtfA), KDP390 (gtfB), W50 (wild type), W50 PorR (porR), and W50 WbpB (wbpB). Lane M, molecular marker; lane MG, molecular marker for glycoproteins; lanes P, whole-cell lysates prior to application to the column; lanes B, proteins bound to the WGA column.