FIG 9.

Relative growth inhibition of heterologous versus homologous parasite lines. Shown is IgG-mediated growth inhibition of the W2mef parasite line relative to 3D7 (A) and FVO (B) parasites. The average growth inhibition calculated from duplicate wells from a representative experiment is reported for each rabbit. The means ± standard errors of the means of data for each immunization group are shown where appropriate. IgG concentrations resulting in 40 to 90% inhibition of the 3D7 and FVO parasite lines and >20% inhibition of W2mef parasites were used for this analysis. Failure to meet these parameters resulted in exclusion of two rabbits from the 3D7/FVO group, one rabbit from the 3D7 Ala/FVO Ala group, and five rabbits from the 3D7 Ser/FVO Ser group. *, P < 0.05; **, P < 0.01 (determined by a Mann-Whitney test [one tailed] comparing each combination of mutants with the wt immunization group).