FIG 6.
Surface protein vaccine inhibits bacterial replication in leukopenic mice with Staphylococcus aureus bloodstream infection. Mock-immunized (closed circles) or vaccinated (ClfA, FnBPB, SdrD, and SpAKKAA) (open circles) leukopenic (cyclophosphamide-treated) CD-1 mice were infected with 2 × 106 CFU of S. aureus Newman as described in the legend to Fig. 5. Animals were euthanized 3 h (A), 6 h (B), 1 day (C), or 3 days (D) postchallenge. Kidney, lung, and liver tissues were removed during necropsy and analyzed for staphylococcal load by plating serially diluted samples on agar plates and enumerating CFU. Statistical significance of differences in bacterial loads between mock-treated and vaccinated leukopenic mice was analyzed with the Mann-Whitney test; P ≤ 0.05, significant difference.