FIG 2.

Localization of B. cenocepacia and P. aeruginosa in CF lungs removed during transplantation. (A) Low-power view of a bronchus from a 32-year-old male whose last preoperative sputum culture was positive for B. cenocepacia and negative for P. aeruginosa (patient 10). The luminal mucopurulent secretions react strongly with Bcc antiserum, with more densely stained areas but without obvious colonial associations. (B) Higher-power view of panel A illustrating predominantly intracellular material in phagocytes and occasional intercellular individual bacteria or pairs/small clusters of bacteria. (C) Low-power view of a bronchus from a 28-year-old female (patient 21) (Table 1) whose last preoperative sputum culture was positive for P. aeruginosa and negative for Bcc bacteria. There are abundant ball-like bacterial colonies and sheet-like masses in the luminal secretions that react strongly with P. aeruginosa antiserum. (D) Higher-power view from panel C illustrating bacterial colonies.