FIG 4.

Aerobic and anaerobic growth of Bcc bacteria in modified M9 medium. To test for strategies used by Bcc bacteria to gain energy anaerobically, we developed a test medium (modified M9 medium), which was supplemented with the electron acceptors nitrate, sulfate, and arginine. B. multivorans J-1 and B. cenocepacia BC-7 (approximately 200 to 500 CFU/1 μl PBS) were incubated for 72 h under aerobic and anaerobic conditions in 30 μl of test medium (n = 3), and the number of bacteria was determined by serial dilutions and plating.