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Growth of Bcc bacteria in mucin gels. Purified mucins from bovine cervical mucus were suspended in PBS, and the mucin gels were incubated with B. multivorans J-1 and B. cenocepacia BC-7 (approximately 200 to 500 CFU/1 μl PBS) for 72 h under anaerobic conditions (n = 3). Enumeration was performed by serial dilutions (see also Fig. S1 in the supplemental material).
