SEM images of S. aureus biofilms cultivated on silicone coupons for 1 day plus 16 h in growth medium alone (A, B) or for 1 day in growth medium followed by 16 h in medium supplemented with 0.35 mM GML (C, D). (A) Low magnification of a control biofilm without GML containing areas of staphylococcal cells covered by relatively homogenous and smooth matrix material; (B) higher magnification of the area highlighted in panel A; (C, D) more irregular and porous appearance of the matrix associated with GML-treated biofilms. Scale bars, 10 μm (A) and 2 μm (B, C, D).