Effects of iron chelators on cellular iron and cell cycle progression. (A) Chemical structures of Dp44mT, Bp4eT, and Bp4aT. (B to D) General steps for synthesis of PPY (control) and the PPYeT and PPYaT iron chelators. (E to G). Nano-LC-FTMS analyses of PPY, PPYeT, and PPYaT. TIC, total ion current; EIC, extract ion chromatogram; PA, peak area. As shown in the EICs, PPY could also be detected in the samples of PPYeT and PPYaT. The purities of PPYeT and PPYaT were 85% and 90%, respectively.