FIG 3.

Inhibition of one round of HIV-1 replication and toxicity in CEM T cells and PBMCs. (A and C) CEM T cells (A) or PBMCs activated with PHA and IL-2 (C) were infected with VSV-G-pseudotyped pNL4-3.Luc.R−E− (HIV-1 Luc) virus for 18 h at 37°C and then treated for 24 h at 37°C with the indicated concentrations of iron chelators. The cells were then lysed, and luciferase activity was measured. EC₅₀s were determined with GraphPad Prism 6 software. (B) CEM T cells were treated with the indicated concentrations of iron chelators for 24 h at 37°C. CEM T cells were treated with 0.4 μM calcein-AM for 30 min, and calcein fluorescence was measured at a 485-nm excitation wavelength and 515-nm emission wavelength on a luminescence spectrometer equipped with a robotic arm (PerkinElmer LS 50B). EC₅₀s were determined with GraphPad Prism 6 software. (D) Activated PBMCs were treated with the indicated concentrations of iron chelators for 24 h at 37°C, and the viability of cells was measured by the trypan blue exclusion method. Fifty percent cytotoxicity concentrations (CC₅₀s) were determined with GraphPad Prism 6 software.