FIG 4.

Effects of SQ109 and AU1235 on the biosynthesis and export of sulfolipids, di- and polyacyltrehaloses, and phthiocerol dimycocerosates in M. tuberculosis. Lipid analysis of untreated, SQ109-treated, and AU1235-treated M. tuberculosis H37Rv mc²6206 cells. Surface-exposed (S) and cell-associated (C) [¹⁴C]acetate-labeled and [1-¹⁴C]propionate-labeled lipids from bacterial cultures treated with SQ109 or AU1235 or untreated were analyzed by TLC in the solvent systems (CHCl₃/CH₃OH/H₂O [20:4:0.5]) ([¹⁴C]-acetate-labeled lipids) (a), (CHCl₃/CH₃OH/H₂O [60:30:6]) ([1-¹⁴C]propionate-labeled lipids) (b), (petroleum ether [60/80°C]/ethyl acetate [98:2]; three developments) ([1-¹⁴C]propionate-labeled lipids) (c), first dimension: (petroleum ether [60/80°C]/acetone (92:8); three developments); second dimension: (acetone-toluene [95:5]) ([1-¹⁴C]propionate-labeled lipids), and revealed by autoradiography (d). The same volume of samples was loaded per lane. The amount of radioactivity incorporated in SL-I, the diacylated sulfolipid precursor (Ac₂SL), DAT, PAT, and PDIM in the treated and untreated cells was semiquantified using a PhosphorImager, and the results are presented in Table 3. CL, cardiolipin; PE, phosphatidylethanolamine.