FIG 4.

WC5 inhibits the binding of IE2 to the MIEP crs wt element. Electrophoretic mobility shift assays (EMSAs) were performed to evaluate the effects of WC5 and WC5E on the IE2DN290-crs interaction by incubating the 6His-IE2DN290 protein with MIEP crs-containing probes either wt (A) or mutated (mut) (B) in the absence or presence of WC5 and WC5E. The binding reaction products were then resolved on 5% native polyacrylamide gels and stained with GelRed. The down-facing arrow indicates the absence of the protein-probe complex in the presence of WC5.