FIG 7.

WC5 inhibits the IE3-dependent transactivation of the early E1 gene promoter of MCMV. NIH 3T3 cells were transfected with a plasmid containing luciferase reporter gene under the control of the E1 promoter (pE1-Luc) or cotransfected with an IE3-expressing plasmid (pIE3) and then treated with 0.2% DMSO, 50 μM WC5, WC5E, or GCV. The reported values represent the means ± SD from three independent experiments in duplicate and are expressed as RLU. *, P < 0.05 versus calibrator sample (pIE3 plus DMSO).