FIG 3.

Effects of oxidants on mitochondrial dGK and TK2 protein levels. Approximately equal numbers of U2OS cells were incubated with various concentrations of H₂O₂ (A) or 1 mM tBHP (lane 2), 2 mM diamide (lane 3), or no treatment (lane 1) (control) (B) for 30 min at 37°C. Mitochondrial extracts were prepared, and 30 μg protein was subjected to 12% reducing SDS-PAGE. The endogenous TK2 protein levels with the tBHP and diamide treatments and the dGK protein levels in all groups were measured by Western blot analysis. The COX IV levels were used as the mitochondrial loading control. The experiments were repeated at least three times, and one representative blot is shown.