FIG 3.

Effect of TFV on MIP-3α, IL-8, and TNF-α in CD4⁺ T cells and CD14⁺ cells. CD4⁺ T cells were isolated from Em, Cx, and Ecx, and CD14⁺ cells were isolated from Em. Cells were treated with 1 mg/ml of TFV for 24 h. RNA was isolated, and RT-PCR was performed to compare the levels of mRNA of MIP-3α, IL-8, and TNF-α. mRNA was expressed as a relative expression compared to untreated samples. Secretions of MIP-3α and IL-8 were analyzed from culture supernatants by ELISA. Each TFV-treated sample was compared with its own control, and fold changes in protein secretion were calculated by converting each control to 1. Bars represent means ± SEM from 6 to 8 independent experiments with different patients. *, P < 0.05; **, P < 0.01; ***, P < 0.001. (A) Expression of MIP3α, IL-8, and TNF-α from Em, Cx, and Ecx CD4⁺ T cells in the presence of TFV; (B) secretion of IL-8 and TNF-α from Em, Cx, and Ecx CD4⁺ T cells; (C) expression of MIP3α, IL-8, and TNF-α from CD14⁺ cells from Em in the presence of TFV; (D) secretion of MIP3α, IL-8, and TNF-α from CD14⁺ cells from Em in the presence of TFV.